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Gram's crystal violet 500ml - Merck

MERCK
Gram's crystal violet 500ml - Merck

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MOD: 25174-00
In Stock
  • Price: 51.20€
  • 41.29€ + TAX 24%

Brand:MERCK

Description

Gram's crystal violet 500ml - Merck 

The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative.
 
Themureine structure of the bacteria walls is the basis of the color affinity.
 
Bacteria will be stained with Gram'scrystal violet solution - an aniline dye - in the first step.
 
After the treatment with iod solution (Lugolʼs solution), a dye-iod complex will form.
 
During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue.
 
Gram-negative bacteria have monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorised .
 
Gram-negative bacteria will be counterstained by Gram's safranin solution and appear
orange.
 
A bottle with 500 ml Gram's safranin solution is sufficient for staining up to 250 slides.
 
it is also available as part of the Gram-color stain set.
 
It is an IVD product and CE registered
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